Efficient transduction of myeloid cells by an HIV-1-derived lentiviral vector that packages the Vpx accessory protein
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چکیده
منابع مشابه
Efficient gene transduction to cultured hepatocytes by HIV-1 derived lentiviral vector.
HEPATOCYTE transplantation can be considered a viable and effective replacement therapy for enzymedeficient liver diseases as well as a supportive therapy for many forms of liver failure. However, genetic modifications of hepatocytes in an ex vivo manner may further advance the field of hepatocyte transplantation. A proofof-concept study by Grossman et al demonstrated the clinical feasibility o...
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The function of dendritic cells (DCs) in the immune system is based on their ability to sense and present foreign antigens. Powerful tools to research DC function and to apply in cell-based immunotherapy are either silencing or overexpression of genes achieved by lentiviral transduction. To date, efficient lentiviral transduction of DCs or their monocyte derived counterparts (MDDCs) required hi...
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The SAMHD1 triphosphohydrolase inhibits HIV-1 infection of myeloid and resting T cells by depleting dNTPs. To overcome SAMHD1, HIV-2 and some SIVs encode either of two lineages of the accessory protein Vpx that bind the SAMHD1 N or C terminus and redirect the host cullin-4 ubiquitin ligase to target SAMHD1 for proteasomal degradation. We present the ternary complex of Vpx from SIV that infects ...
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Objective(s): Neural stem/progenitor cells (NS/PCs) hold a great potential for delivery of therapeutic agents into the injured regions of the brain. Efficient gene delivery using NS/PCs may correct a genetic defect, produce therapeutic proteins or neurotransmitters, and modulate enzyme activation. Here, we investigated the efficiency of a recombinant lentivirus vector ...
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ژورنال
عنوان ژورنال: Gene Therapy
سال: 2012
ISSN: 0969-7128,1476-5462
DOI: 10.1038/gt.2012.61